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After preincubation, the samples were placed on ice and other components were added to the mixture: 4 L 5 concentrated RT buffer (250 mM Tris-HCl, 250 mM KCl, 20 mM MgCl 2 , 50 mM DTT, pH 8.3 at 25 C), 2 L deoxyribonucleotide triphosphates (dNTPs, 10 mM) and 1 L RNase inhibitor (20 U/L) and 1 L GoScriptTM Reverse Transcriptase (160 U/L) in a total volume of 20 L

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Elevated oxidation of docosahexaenoic acid, 22:6 (n-3), in brain regions of rats undergoing ethanol withdrawal

Run together, they produce a synergistic pulse larger than either alone