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They are not approved for human or animal use nor consumption by the Food and Drug Administration (FDA)

Mutations in FOXO genes have been suggested to cause hyperglycemia and hyperlipidemia and may even increase the risk of carcinogenesis in test models

However, because excessive copper exposure can be harmful, research protocols monitor total copper levels, especially in systemic studies

If required, cells were transfected with plasmid DNA using jetPRIME according to the recommended protocol of the supplier, one to two days before imaging