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If pain or gut symptoms are affecting your daily life
Rationale: Continuous exposure can downregulate receptors or alter endogenous hormone feedback
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Binding/Wash Buffer: PBS, pH 7.4 (140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH 7.4) Elution Buffer: 50 mM Tris-HCl, 10-20 mM reduced glutathione, pH8.0 2.2 Preparation of the Magnetic Beads 1) Completely resuspend the beads by shaking or vortexing the vial
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