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The NGs were treated 20 min at 37 C with 0.10.5 mg ml 1 collagenase Ia (Sigma), 0.40.6 mg ml 1 dispase II (Roche, Basel, Swiss), 15 g ml 1 DNase II type IV (Sigma), and 0.75 mg ml 1 bovine serum albumin (Sigma) in HKRB supplemented with 5.6 glucose

(ii) Cu 2+ induces the secretion of acidic SMase from leukocytes which in turn leads to accumulation of erythrocytic ceramide [131] and (iii) treatment of erythrocytes with extracellular SMase resulted in ceramide-associated alterations in membrane-cytoskeleton interactions and membrane organization [125]

the refined peptide solution was transformed into salts using Waters 2545 RP-HPLC system with a chromatographic column of 50 x 250 mm reverse phase C18 column and 0.1% trifluoroacetic acid/acetonitrile as a mobile phase

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