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For compound treatment, the medium was replaced by 90% of Williams E Medium supplemented with 12.5% HepaRG preinduction and toxicity medium supplement, 0.6% HepaRG induction medium supplement (Lonza, catalog: MHIND), and 25 U/mL penicillin-25 g/mL streptomycin

They offer fast exposure but require precise pH and droplet size control to ensure consistency

According to a report in Scientific World Journal , Toxic metals such as arsenic, cadmium, lead, and mercury are ubiquitous, have no beneficial role in human homeostasis, and contribute to noncommunicable chronic disease. Researchers have identified that significant exposure to at least 23 different environmental metals (called heavy metals) can contribute to acute or chronic toxicity

This suggests a protective mechanism against Mn overload in neonates, a vulnerable developmental stage (Yu and Zhao, 2023)