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A major limitation of this study was that it relied on parent recall to assess whether advice was given.14 The use of parent recall in research has the advantage of being realistic by assessing whether the parents perceived that they were given advice, but the findings more likely speak to the need for better ways to provide education to parents

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qPCR (20 L) was carried out by using 2 Ultra SYBR Mixture (CWbio, China) in 12 8 well plates in a CFX Connect Real-Time System (Bio-Rad), containing 10 L of SYBR Mixture, 2 L cDNA, 1.6 L of qPCR primers (4 M), and 7.4 L nuclease-free water

Foresman JB, Fox DJ