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Methods Construct cloning The full-length human ghrelin receptor (residues 1366) was sub-cloned into pFastBac1 vector with an N-terminal haemagglutinin signal peptide (HA) and His10 tag followed by BRIL epitope, as well as LgBiT at the C-terminus to facilitate the protein expression and stability

Delivery of therapeutics Viral delivery of CRISPR-Cas9 gene constructs and ASOs have been a commonly used method that utilizes vectors such as lentiviruses, adenoviruses, and adeno-associated viruses (AAV) [141] (Table 1)

Targeted metabolic profiling of pomegranate polyphenols and urolithins in plasma, urine and colon tissues from colorectal cancer patients

They are crucial for the establishment of a functional Th17 response after the infection in the gut (with the help of IL-2 ) [ 45 ]