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Some researchers split the powder between two reconstitution sessions to work around this limitation, though this requires advanced technique and introduces additional contamination risk

Philp and colleagues (FASEB J, 2003) showed the LKKTETQ actin-binding site alone is sufficient to promote endothelial cell migration and angiogenesis, and Bock-Marquette and colleagues (Nature, 2004) reported activation of integrin-linked kinase and Akt with cardiomyocyte and epicardial cell migration in mouse cardiac injury models, indicating a signalling role separate from actin buffering

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Researchers who maintain this consistent concentration across vial sizes eliminate a major source of dosing errors