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[15] The redox potential (E GSH ) was calculated using the following equation: Where E (roGFP2) is the standard redox potential of the fluorescent protein (280 mV, at pH 7), [12] R is the gas constant (8.315 J K -1 mol -1 ), T is the temperature (K), and F is the Faraday constant (96485 C mol -1 )

(Spironolactone is not a loop diuretic.) In this 2010 European study, spironolactone, when added to the conventional CHF therapies, was found to significantly decrease the risk of reaching the primary endpoint (i.e., cardiac-related death, euthanasia, or severe worsening) in dogs with MVD

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Adesanya AW, Morales MA, Walsh DB, Lavine LC, Lavine MD, Zhu F