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Freeze-thawed liposomes involve quick freezing and gradual thawing of SUVs followed by sonication of the aggregates which disperses them resulting in the formation of LUVs [144]

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This commercial treatment available in Slovenia was a liquid food supplement (10 mL) containing 4 g of fish collagen, 50 mg of CoQ10, 80 mg vitamin C, and 920 g of biotin administered for 12 weeks, once per day ( n = 17 placebo control