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The replication of minicircle DNA is a complex mechanism that begins with the attachment of the universal minicircle sequence (UMS), located at the origin of replication of minicircle DNA, with the UMS binding protein (UMSBP), firstly identified in Crithidia fasciculata (a related, non-pathogenic trypanosome) (Tzfati et al., 1992) and more recently in T