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Six randomly chosen plants per treatment (four mature leaves per plant) were taken and cut into 1 cm segments

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Additional studies showed that the rapid androgen action involves a pertussis toxin (PTX) sensitive-G (Gi/Go) protein-linked receptor at the plasma membrane and leads to IP3-mediated calcium signaling the Ras/mitogen-activated extracellular signal-regulated kinase (MEK)/ extracellular signal-regulated kinase (ERK) pathway activation as well in muscle cells [88]