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Sample Preparation Desalting and buffer exchange Enzymatic digestion for peptide mapping Minimizing peptide adsorption and degradation Proper sample preparation helps prevent contamination and reduces signal suppression during LC-MS analysis

Semaglutide and tirzepatide allow for albumin binding and resistance to DPP4 degradation, creating a significantly longer half-life (5 days for tirzepatide and 7 days for semaglutide) and thus a greater effect on receptors and downstream pathways in the central nervous, cardiovascular, digestive, and musculoskeletal systems as described below [37, 38]

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