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All animals were housed at standard temperature (~22C) and humidity-controlled conditions under a 14L:10D photoperiod and provided ad libitum access to water and a low-fat-purified diet upon arrival (10% calories from fat D12450H Research Diets Inc)

2.2.2 Thermal stress of myocytes Myocytes were seeded at a density of 110 6 per well in a 24-well plate, and subjected to high-temperature culture at 37C for 2, 4, 6, 12, and 24 hours