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Together these modifications dramatically reduce binding to IGF-binding proteins (IGFBPs), extending the free fraction half-life from approximately 10-15 minutes for native IGF-1 to roughly 20-30 hours for IGF-1 LR3

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The FDA recommends consumers, healthcare providers, and supply chain stakeholders buy safe, effective, FDA-approved medicines from state-licensed pharmacies

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However, these assays do require acid-ethanol precipitation or addition of excess IGF-2 to minimize binding protein interference (Guha et al., 2013)

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