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Non-genetic predictors of BMI loss To analyse the dependence of achieved BMI loss on non-genetic factors such as drug type, dosage and time on treatment, we fit the following model: where drugType is an indicator variable that equals 1 for individuals using semaglutide and 0 for tirzepatide, dose represents the dose in milligrams, days treat represents the total days on the relevant drug and : represents an interaction term between two or more variables

Finally, plates were read at 450 nm on a BioTek Synergy HT plate reader using BioTek Gen5 (BioTek)

This figure shows predicted BMI % compared to observed values, in deciles, as measured in the held-out test set