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Following treatment, cells were washed with PBS and then incubated with 500 L DCFH-DA working solution (20 M) at 37 C for 30 min

Guo, S., Yang, L., Wang, G., Asea, A

Remember that hormone optimization is highly individual

(e) cross-sectional studies with no time restriction, (f) only studies published in English were included due to resource limitations and to ensure accuracy in data extraction and risk-of-bias assessment