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Current investigations additionally evaluate signaling pathways involving nitric oxide synthase (NOS) , vascular endothelial growth factor (VEGF) , fibroblast growth factors (FGFs) , transforming growth factor-beta (TGF-) , actin-binding proteins, extracellular matrix proteins, and intracellular regulatory molecules associated with peptide-mediated communication

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Protein expression was induced by addition of 0.1 mM IPTG and cultured for an additional 16 18 hrs at 25 C