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Dissected cochlear epithelia are transferred to permeable membrane inserts (Millicell organotypic culture plate inserts, Millipore) which are placed into 1 mL of culture media (Dulbecco's modified Eagle's medium (high glucose, Glutamax, 25 mM HEPES) with 10% fetal bovine serum, 1% N2 supplement and 10 units/ml penicillin) in a 6-well plate
Although the nation remains ill-equipped to manage a cyanide disaster, significant progress is being realized in some aspects of preparedness
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Financial support and sponsorship This work was supported by grants from the National Science Foundation ( IOS-1557341 (HFN, MCR) and DMS-2038056 (AC)
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