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Upper airway disease needs to be taken more seriously with accurate diagnosis of AR, since it is likely that, if effectively treated early, some asthma may be prevented
A significant focus was on developing inhibitors of insulin-regulated aminopeptidase (IRAP), a protein identified as the AT4 receptor, with the goal of enhancing metabolic stability and BBB permeability
Samples were added to membranes from custom rodent antibody arrays (Abcam, Cambridge, UK) containing specific capture antibodies for C-reactive protein, active GLP-1, insulin, adiponectin, and leptin, and allowed to incubate overnight before being retrieved and incubated again with a second antibody array membrane
This provided an ideal setting to begin his hunt for the putative GIP
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