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Pathological human astroglia in Alzheimers disease: opening new horizons with stem cell technology

NgoBVan RiperJCantleyLYunJ

Following SDS-PAGE, a 1 mm circular slice was picked from the gel using an Ettan Robotic Spot-Picker and submitted for proteolytic digestion (Trypsin) and peptide extraction at the Functional Proteomics Facility at the University of Western Ontario

Further validation by western blot revealed that the level of JAK2 and STAT4 in the shGSTM3 were enhanced significantly than that in the shNC, and vice versa in the GSTM3-OE (Figure 8E)