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Furthermore, while enzymatic removal of the L585 residue decreased FcRn binding for fusion designs with WT albumin, this was not the case for the corresponding QMP-engineered variants

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According to the standard procedure, 30 mg tissue samples were lyzed with 500 L of cold RIPA lysis buffer, lysates were collected, and the total protein content was estimated using a BCA protein assay kit (Sigma, St