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A wide range of studies reported both overt and subclinical hypothyroidism as a risk factor of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH), the chronic liver diseases that might progress to cirrhosis and hepatocellular carcinoma [3741although some newly published studies failed to find such an association [42,43]

A small number of people are allergic to B12, but this is rare

Following the transfer, the membrane was incubated with the aforementioned primary antibodies, and detection was achieved using appropriate secondary antibodies and chemiluminescent substrates