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Successful transconjugants (containing a chromosomally integrated plasmid after the first homologous recombination event HR1) were selected by incubation on LB plates supplemented with kanamycin
Common brands include semaglutide (Ozempic and Wegovy) and tirzepatide (Mounjaro)
Activation of the GLP-1R by GLP-1 also results in recruitment of -arrestin-1 (Arr1) and -arrestin-2 (Arr2), which mediate receptor internalization and may serve as scaffolds for G protein-independent signaling ( 18, 19 )
[2] [4] Common side effects include nausea, pain at the site of injection, and headache
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