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Annual initiation prevalence was calculated for each eligible BMI subgroup

Injectable anti-aging protocol: Dose: 1-2mg daily Frequency: 5 days on, 2 days off OR daily for 30 days Cycle: 30 days on, 14 days off Duration: Minimum 2-3 cycles for substantial results Topical anti-aging protocol: Concentration: 2-3% serum or cream Application: Twice daily (morning and evening) Duration: 12+ weeks continuous use Combine with: Sunscreen (always), retinoids (evening, separate application) Combination approach: Maximum anti-aging results come from combining injectable and topical

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CYP2E1 and ALDH enzymatic activity CYP2E1 activity was assessed by measuring the rate of oxidation of p -nitrophenol (PNP) to p -nitrocatechol as described 54