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Interestingly, our results are consistent with other studies showing that repeated sulforaphane treatment could result in a long-term elevation of free radical defence, though the sustained elevation of expression of Nrf2, HO-1 and NQO1 seems unlikely 27,46,47

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Biotinylated goat anti-mouse IgG or goat anti-rabbit IgG (Vector Labs, BA-9200 1:200 and BA-1000 1:200, respectively) secondary antibodies were added in blocking buffer and incubated for 1 h at room temperature