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All protocols are supervised by Dr

DepMap is a comprehensive resource that contains genome-wide CRISPR-Cas9 and shRNA screens in over 1,000 cell lines and calculates gene effect score for individual genes in each cell line

Primary sites: Abdomen (avoiding 2 inches around the navel) Outer thighs Back of upper arms Upper buttocks Rotation pattern example: Day 1: Left abdomen Day 2: Right abdomen Day 3: Left thigh Day 4: Right thigh Day 5: Left arm Day 6: Right arm Day 7: Return to left abdomen Keep at least one inch between injection sites in the same general area

The concentrations of glutathione were then calculated from slopes of the absorbance changes in reference to the standard curve