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Applications of biotin ligase-based proximity marker technology Biotin ligase-dependent proximity labeling techniques have been widely applied in proteinprotein interaction studies and in determining the proteomic localization of cellular structures, including nuclear pore complexes, transcription regulatory complexes, Hippo pathway components, stress granules, cilia, centrosomes, pathological protein aggregates, and interactions with viruses and pathogens [33, 66, 77,78,79,80,81]

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