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Asolectin (25 mg/ml) was homogenized in 50 mM KH 2 PO 4 -KOH (pH 8.0), 20 mM KCl, purged with nitrogen and sonicated (Vibracell Ultrasonic liquid processor VCX130, Sonics) on ice, without the use of detergent, until the solution became optically clear

subtilis strain RB 50 is performed on carbon-limited fed-batch cultivations at a 35 m 3 scale and has a short cycle time (4856 h) (Hohmann et al., 2001)

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