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Different fluorescently labeled cDNA probes were mixed with 30 L of hybridization buffer (3 SSC, 0.2% SDS, 5 Denhardt's solution, and 25% formamide) and applied to the microarray (CapitalBio human mRNA microarray V2.0, CapitalBio, Beijing, China) followed by incubation at 42 C for 16 hours

Dietary intake remains the main source of selenium, which has prompted considerable attention toward enriching foods with this nutrient