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We circumvented the need for CYP-mediated bioactivation of AQ and DEAQ by exposing the cells to the synthetic AQ-QI and DEAQ-QI, the reactive metabolites of AQ

Choline serves as the precursor for the synthesis of phosphatidyl choline (PC), the main phospholipid in brain, liver and other tissues

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These results validate LNPs as an effective tool for specifically delivering DNA vector to the liver, supporting their use in further in vivo genome editing experiments