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This heightened permeability facilitates the translocation of bacterial endotoxins, such as LPS, into the systemic circulation

LDH assay Non-specific membrane disruption was assessed quantitatively by measuring the lactate dehydrogenase (LDH) activity in the medium using the cytoscan-LDH cytotoxicity assay kit (G-Biosciences Inc., St Louis, Mo, USA)

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the STEP TEENS trial showed weight rebound after discontinuation (as in adults), suggesting that many patients may need extended or intermittent treatment