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Then, all animals were euthanized by bleeding after transection of the abdominal vena cava under anesthesia and the liver, heart, soleus muscle, gastrocnemius muscle, tibialis anterior muscle, extensor digitorum longus muscle, epididymal white adipose tissue, and brown adipose tissue were harvested and immersed in RNAlater solution (Qiagen, Venlo, Netherlands) for qPCR analysis

Key Safety Checkpoints Before Starting GLP-1 Therapy Before initiating any GLP-1 medication, Crohn's patients must confirm disease remission or minimal inflammation through recent lab work (CRP, fecal calprotectin) and imaging if available

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