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For peptides that do not dissolve cleanly in bacteriostatic water alone typically those with hydrophobic sequences or basic amino acid-rich compositions that require protonation for solubilisation the recommended approach is to first perform initial reconstitution in a small volume of 0.1% acetic acid water (also available from Peptides Lab UK) to produce a clear stock solution, before diluting further with bacteriostatic water or the relevant physiological buffer

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To further elucidate the biological functions and signaling pathways of intersection genes, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted using the clusterProfiler R package