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The assessment of parasite viability microscopically in vitro is based on regular lack of movement of larvae (motility) and morphological changes such as granularity and shape alterations [37] that might be subjective and inaccurate [41]

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In contrast, an overdose of APAP causes hepatotoxicity by saturating the sulfation pathway and generating NAPQI that leads to a robust reaction between NAPQI and hepatic glutathione stores, which subsequently depletes glutathione (Xie et al., 2015)