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The receptor-bound HPC4 resins were washed with a buffer containing 20 mM HEPES pH 7.5, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG, 0.006% (w/v) CHS, and 5 M semaglutide supplemented with 5 mM CaCl 2 before the bound material was eluted in a buffer supplemented with 10 mM EDTA

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Regular review of weight loss progress, typically every three months, allows assessment of treatment response and consideration of whether continuation is appropriate according to NICE TA875, which recommends discontinuation if less than 5% weight loss is achieved after six months at the maintenance dose

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